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KMID : 1189420090220030063
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2009 Volume.22 No. 3 p.63 ~ p.70
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Anti-inflammaory effects of the MeOH extract of Gentianae Macrophyllae Radix in vivo
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Abstract
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Objectives : The present study was examined to evaluate the anti-inflammatory effects of the Gentianae Macrophyllae Radix MeOH extracts (GMR) in vivo.
Methods : The effects of GMR on anti-inflammation were measured by production of NO, TNF-α (Tumor Necrosis Factor-alpha) and IL-1β (Interleukin-1β), IL-6 in Raw 264.7 macrophage cells stimulated with LPS.
Results : 1. All concentrations of GMR(0.10 ㎎/㎖) had no significant cytotoxicity in Raw 264.7 cell during the entire experimental period. 2. The level of NO and iNOS in culture medium was dramatically increased by LPS application. However, these increases were dose-dependently(0.03 and 0.10 ㎎/㎖) attenuated by treatment with GMR. 3. All concentrations of GMR significantly inhibited the production of IL-1β in Raw 264.7 macrophage cells stimulated with LPS.
Conclusions : These results provide evidences that therapeutic effect of GMR on heat syndrome, especially due to the acute inflammation, are partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of p-IκBα. Moreover, it suggests that the mechanism of action of GMR comes from the suppression of inflammatory mediators, such as NO, PGE2 and pro-inflammatory cytokines.
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KEYWORD
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Gentianae Macrophyllae Radix, Anti-inflammaory effects, NO, TNF-α, IL-1β, IL-6
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